Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway

Figure 5

miR-34a-3p downregulates CAB39 expression. (a) Relative expression levels of miR-34a-3p and (b) CAB39 were determined by RT-qPCR in aging DPSCs transfected with miR-34a-3p mimics or miR-NC for 48 h followed by 100 μM metformin for 48 h. (c) Representative blots and (e) quantitative analysis of CAB39 protein expression levels in aging DPSCs transfected with miR-34a-3p mimics or miR-NC for 48 h followed by 100 μM metformin for 48 h. (d) The predicted potential binding site of miR-34a-3p at the 3UTR of CAB39 in the human gene is shown. Meanwhile, a mutation was constructed where the letters in red indicate mutated nucleotides. (f) Relative luciferase activity. Data are expressed as (). NS: not significantly different. , with comparisons indicated by lines.

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