Review Article
Extracellular Vesicles: Novel Roles in Neurological Disorders
Table 1
Comparison of different isolation methods of exosomes.
| Isolation technology | Separation principle | Sample size | Advantages | Disadvantages | Ref |
| Ultracentrifugation | Molecular size, density, and shape | Large | Low risk of pollution, low reagent cost | Expensive equipment, time-consuming, poor biological activity, and integrity of exosomes | [22] | Size exclusion chromatography | Molecular size | Medium | Yield, purity, integrity, and biological activity of exosomes can be ensured | Special equipment | [23] | Immune-affinity capture | Specific binding of antigen and antibody | Small | High purity | High cost, low yield | [24] | Ultrafiltration | Molecular size and shape | Large | Efficient and convenient | Low purity, exosomes may partially remain on the membrane | [25] | Microfluidic | Immune affinity, size, and density | Small | Fast, low cost, convenient, and automated | The selectivity and specificity need to be verified | [27] |
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