Research Article

Long Noncoding RNA Expression Profiles of Periodontal Ligament Stem Cells from the Periodontitis Microenvironment in Response to Static Mechanical Strain

Figure 5

The expression levels of DElncRNAs in HPDLSCs and PPDLSCs during osteogenic differentiation: (a) the expression levels of DElncRNAs in HPDLSCs and PPDLSCs after osteogenic differentiation for 7 days; (b) the expression levels of lncRNA-XIST in strained HPDLSCs and PPDLSCs; (c) detection of the osteogenic gene Runx2 in strained HPDLSCs infected with shlncRNA-XIST; (d) detection of the osteogenic gene Runx2 in strained PPDLSCs infected with lncRNA-XIST; (e) alizarin red staining for HPDLSCs and PPDLSCs; (f) calcium quantification for HPDLSCs and PPDLSCs. All experiments were performed in triplicate, and the data are presented as the Scale μm.
(a)
(b)
(c)
(d)
(e)
(f)