The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism
Influence of differently treated FBS on MSC behaviour. Analyses of hMSCs cultivated in media with heat-inactivated FBS (iFBS) and heat noninactivated FBS (non-iFBS) after 48 h of cultivation (a, b) or 14 days (c) or 21 days (d): (a) metabolic activity; (b) doubling time; (c) CFU-F and colony morphology (white bars correspond to 232 μm); (d) osteo-differentiation of hMSCs cultivated in osteo-differentiation media supplemented with iFBS and non-iFBS (used von Kossa staining) ((a) Analysed in 5% iFBS and 5% non-iFBS; (b–d) analysed in 10% iFBS and 10% non-iFBS). Statistical analysis was done by one factor ANOVA () with post hoc Fischer LSD test. Significant difference () between iFBS and non-iFBS (95% confidence interval was determined to verify the similarity/comparability of samples with a nonsignificant difference).