The Impact of Various Culture Conditions on Human Mesenchymal Stromal Cells Metabolism
Metabolic activity of MSCs cultivated in differently supplemented media. (a) Cells in the media supplemented with 5% allogenic human serum (allo-HS), 5% autologous human serum (auto-HS), and 5% human serum derived from donor of the AB blood group (AB-HS) (results compared to % of non-iFBS). All the results were significantly different to non-iFBS (). (b) Metabolic activity of MSCs cultivated in media with non-iFBS, heat inactivated adult bovine serum (iABS), or noninactivated adult bovine serum (non-iABS). #Significant difference between non-iFBS and samples (). (c) Cells in the media supplemented with 5% heat inactivated FBS (iFBS), 5% human AB serum (AB-HS), and 5% or 7.5% platelet releasate (PR-SRGF) supplement after 4–48 h (results compared to % of non-iFBS). NS: nonsignificant difference to non-iFBS control. Significant difference between samples. #Significant difference between non-iFBS and samples (), based on one factor ANOVA with post hoc Fischer LSD test.