Expression of CD146 and Regenerative Cytokines by Human Placenta-Derived Mesenchymal Stromal Cells upon Expansion in Different GMP-Compliant Media

Figure 2

Effect of PLL on the CD146 expression. The pMSCs were expanded to their second passage in medium as indicated, split, and expanded further for 7 days in the same medium (i.e., LM, serum, serum+PLL, plasma+PLL) or in medium either enriched (>LM + PLL, >serum+PLL) or deprived from PLL (>serum, >serum). (a) The average of CD146 mean fluorescent intensities (MFI) dropped in all experiments when pMSCs were further cultured in medium complemented with PLL (grey bars), while it increased, when pMSCs were further cultured in medium deprived from PLL (black bars). The bars show the from pMSCs of 5 donors. (b) Messenger RNA amounts were enumerated by qRT-PCR in pMSCs expanded in medium either complemented with PLL (grey bars) in comparison to medium without PLL (black bars). In all cases investigated, addition of PLL reduced CD146 transcript amounts, while reduction of PLL from expansion in serum+PLL to serum only caused an increase in CD146 transcripts. The change from plasma+PLL to serum had no effect on CD146 transcript amounts. The bars show the mean normalized transcript amounts from pMSCs of 3 donors.

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