Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing <i>β</i> Cells

<div>Characterization of human-induced pluripotent stem cells (iPSCs). (a) Undifferentiated iPSC colonies. Fully reprogrammed iPSC colonies are round, compact cell aggregates with sharp, distinct borders. Immunostaining of (b) Oct4, (c) Nanog, (d) Sox2, and (e) SSEA4 in undifferentiated iPSCs. (f) Teratoma derived from immunodeficient mice injected with iPSCs. Human-induced pluripotent stem cells from tissues representing embryonic germ layers: pigmented epithelium (ectoderm), cartilage (mesoderm), and glandular structure (endoderm).</div>

Stem Cells International

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Figure 1: Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing <i>β</i> Cells 

Figure 1 | Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing β Cells 