Research Article

miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

Figure 2

miR-129-5p promoted cell proliferation and migration. BMSCs were transduced with a lentiviral vector containing miR-129-5p mimic, miR-129-5p inhibitor, or negative control (NC) lentivirus, respectively. 48 hours after transduction, cells were used for the following analysis. (a) miR-129-5p expression was examined by quantitative RT-PCR. (b) Immunostaining with Ki67 (red) antibody and DAPI (blue) in BMSCs. Scale bars, 40 μm. (c) Quantification of Ki67-positive cells in analysis was shown at (b) (). (d) Western blot analysis showed the expression of Ki67. (e) Quantification of Ki67 protein levels was shown at (d). (f) Cell viability was estimated with CCK-8 assay at days 1, 3, 5, and 7. (g) Images were taken at 0 h and 24 h after scratch wound. (h) Histograms represent relative wound width at 24 h normalized to that at 0 h. Values represent of at least three independent experiments. All values were based on a two-tailed -test. and .
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