miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

<div>miR-129-5p regulated BMSC osteogenic differentiation via repressing Dkk3. (a) pcDNA-Dkk3 or pcDNA-vector was transfected into BMSCs. The expression of Dkk3 was analyzed by western blot. (b) ALP and ARS staining of BMSCs following miR-129-5p mimic transduction or overexpression of Dkk3 during osteogenic differentiation. (c, d) Absorbance index of ALP and ARS was calculated. mRNA (e) and protein (f) levels of osteoblast-related genes Runx2, Bmp2, and OCN were detected by quantitative RT-PCR and western blot, respectively. <i>β</i>-Actin was used as an internal control. (g) Semiquantitative analysis of protein expression levels of Runx2, Bmp2, and OCN. 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Stem Cells International

fig7

Figure 7

Figure 7: miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 