Adventitial Progenitor Cells of Human Great Saphenous Vein Enhance the Resolution of Venous Thrombosis via Neovascularization

Figure 2

Progenitor cells in the thrombi. (a) The result of double staining with Sca1 antibody and CD31 in the 7-day thrombi of mice. The green arrow indicates Sca1⁺ progenitor cells in the venous thrombi. The red arrow indicates CD31⁺ endothelial cells in the intima of venous wall. The double red arrows indicate CD31⁺ endothelial cells of neovascularization within the marginal area of thrombi. The yellow arrow indicates crossing of Sca1⁺ progenitor cells into the endothelial cells within the intima of venous wall. (b) The labeled cells with CD34 antibody in mice. (c) Total cells and positive cells were counted under the microscope. The percentage of progenitor cells in the marginal area of thrombi from 5 mice. (d, e) The result of staining with antibody CD34 in a 25-day thrombus of HGSV. The red dotted line indicates the intima of the vein. The red arrow indicates CD34⁺ cell in thrombus. T: area of thrombi; W: area of vein wall. Scale bars: 25 μm (a, b); 100 μm (d); 50 μm (e).

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