Adventitial Progenitor Cells of Human Great Saphenous Vein Enhance the Resolution of Venous Thrombosis via Neovascularization

Figure 3

The cells outside-in migration into decellularized HGSV. (a) No cell nuclei was visible with HE staining of decellularized vessels. (b) The infrarenal abdominal aorta of rabbit was replaced by decellularized HGSV. The white arrow indicates the patented transplants by ultrasonic examination at 7-day and 14-day replacement. (c) At 7-day replacement, there were a small number of cells in the adventitia of scaffold far from anastomoses; meanwhile, there were several cells emerged from the intima and seldom in tunica media. (d) At 7-day replacement, there were a large number of cells in the adventitia of scaffold close to anastomoses; meanwhile, a large number of cells have emerged in the intima and a few in the tunica media. (e) A large amount of CD34⁺cells in the adventitia of scaffold and a small number of CD34⁺ cells in the intima. (f) Cells entered into the three layers of the scaffold at 14-day replacement. Ad: adventitia of scaffold; W: wall of scaffold; Lu: lumen of scaffold. Scale bars: 25 μm (a); 50 μm (c, d, and f); 100 μm (e). Magnification: 100x (b, c, d, and f); 200x (e).

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