Research Article

Adventitial Progenitor Cells of Human Great Saphenous Vein Enhance the Resolution of Venous Thrombosis via Neovascularization

Figure 4

Isolation and cultivation of CD34+CD117+HGSV-AdPC in vitro. (a) Cells of HGSV wall obtained by collagenase II digestion at 48 hours, 72 hours, and 7 days culture. The green arrow indicates cells growing in colony. (b) FACS isolation of CD34+CD117+HGSV-AdPC; the double positive cells were about ()% in vein wall cells. (c) Immunofluorescent staining with antibodies CD34 (red), CD117 (green), and DAPI (blue) of the FACS-purified HGSV-AdPC after 7 days culture, and the double-positive cells accounted for 91.2%. Scale bars: 200 μm (a), 50 μm (c). Magnification: 100x (a), 400x (c).
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