Research Article

Human Umbilical Cord-Mesenchymal Stem Cells Survive and Migrate within the Vitreous Cavity and Ameliorate Retinal Damage in a Novel Rat Model of Chronic Glaucoma

Figure 6

Characterization of isolated hUC-MSCs. (a) Morphology of hUC-MSCs were recorded at passage 1 (P1) and passage 2 (P2) under a microscope. Scale bars: 200 μm. (b) Immunophenotypic characterization of hUC-MSCs by flow cytometry. The cells were stained with antibodies against CD29-PE, CD44-FITC, CD105-FITC, CD34-FITC, CD45-FITC, HLA-DR-PE, CD80-FITC, CD86-FITC, and CD31-FITC. The hUC-MSCs expressed CD29, CD44, and CD105 but lacked expression of CD34, CD45, HLA-DR, CD80, CD86, and CD31. CD: cluster of differentiation; HLA-DR: human leukocyte antigen-DR isotype; FITC: fluorescein isothiocyanate; PE: phycoerythrin.
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