VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes

Figure 2

Suppression of Nor1 and Akt activity prevents hypertrophy induction in iPS-CM. (a) iPS-CM were seeded on fibronectin-coated coverslips and transfected with Nor1 siRNA or control siRNA, respectively. After 24 h, cells were further treated with PE (100 μM) to induce hypertrophy. iPS-CM cell size was quantified by histological staining of F-actin (red). . (b) iPS-CM were treated with wortmannin (400 nM) before hypertrophy induction by PE. Cell size was quantified by staining of the actin cytoskeleton. Representative images are depicted. Bar: 100 μm. . n.s. = not significant; , .

(a)

(b)