Palmitic Acid Methyl Ester Enhances Adipogenic Differentiation in Rat Adipose Tissue-Derived Mesenchymal Stem Cells through a G Protein-Coupled Receptor-Mediated Pathway

Figure 5

PAME increases adipogenic differentiation through activating the GPR40/120/PLC/ERK pathway. Treatment of rAD-MSCs with PAME (50 μM) in adipogenic induction medium for 12 days enhanced the adipogenic differentiation, and these effects were abolished by cotreatment with U73122, U0126, or BAPTA-AM (a, b). PAME (50 μM) significantly increased the mRNA levels of Pparγ (c) and Gpd1 (d) in rAD-MSCs, and these effects were abolished by cotreatment with U73122, BAPTA-AM, or U0126. These antagonists were dissolved in 0.1% DMSO (vehicle). Values shown in parentheses represent the number in each group. PAME (50 μM) increased the protein levels of PPARγ in rAD-MSCs, and these effects were abolished by cotreatment with GW1100, AH7614, U73122, BAPTA-AM, or U0126 (e) (). All data represent . ^#, versus the AIM group; ^$, versus the AIM+PAME group. AIM: adipogenic induction medium; Veh: vehicle.

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