Research Article

METTL3-Mediated m6A Methylation Regulates Muscle Stem Cells and Muscle Regeneration by Notch Signaling Pathway

Figure 7

YTHDF1 regulates the mRNA translation efficiency of Notch signaling pathway components. (a) The protein levels of Notch signaling pathway in the METTL3 overexpressed or (d) depleted and control cells. (b, e) mRNA expression of Notch signaling pathway analyzed by q-PCR. (c, f) Polyribosome-bound mRNA-qPCR was used to detect translation efficiency. (g) Working model of METTL3 regulates muscle stem cells by Notch signaling pathway. ; ; . NC: negative control; OE: YTHDF1 overexpressed; si: YTHDF1 siRNA.
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