Research Article

Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Carrying MicroRNA-181c-5p Promote BMP2-Induced Repair of Cartilage Injury through Inhibition of SMAD7 Expression

Figure 5

miR-181c-5p represses BMP2-induced SMAD7 expression. (a) Fluorescence microscopy observation of BMSCs after 3 days of infection with BMP2 overexpressing adenovirus (). (b) BMP2 mRNA and protein levels in BMSCs after BMP2 overexpression determined by RT-qPCR and Western blot analysis. (c) SMAD7 mRNA level in BMSCs after BMP2 overexpression determined by RT-qPCR. (d) Fluorescence microscopy observation of BMSCs after 3 days of infection with miR-181c-5p adenovirus (). (e) miR-181c-5p expression after overexpression of miR-181c-5p in BMSCs determined by RT-qPCR. (f) SMAD7 expression in BMSCs after overexpression of miR-181c-5p determined by RT-qPCR. (g) mRNA level of SMAD7 in BMSCs treated with Ad-BMP2 alone or combined with Ad-miR-181c-5p determined by RT-qPCR. (h) Protein level of SMAD7 in BMSCs treated with Ad-BMP2 alone or combined with Ad-miR-181c-5p measured by Western blot analysis. . All experiments were repeated for three times.
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)