Chidamide Suppresses the Growth of Cholangiocarcinoma by Inhibiting HDAC3 and Promoting FOXO1 Acetylation

Figure 3

CDM induces cell autophagy by increasing FOXO1 acetylation level. (a) Acetylation level of FOXO1 in cancer tissues () and normal bile duct tissues () determined by Western blotting. and , compared with normal bile duct tissues. (b) Acetylation level of FOXO1 in QBC939 and SNU308 cells treated with EBSS and CDM-H detected by Western blotting. and , compared with control cells. (c) IP analysis of cells, FLAG precipitation and Ac-FOXO1 immunoblotting. (d) IP detection of QBC939 and SNU308 cells, FLAG precipitation and Ac-FOXO1 nuclear and cytosolic immunoblotting. Data were shown as the . One-way ANOVA or repeated measures ANOVA with Tukey’s post hoc test was used for multi-group data comparison. The experiment was repeated three times.

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