Wharton’s Jelly-Derived Mesenchymal Stem Cells with High Aurora Kinase A Expression Show Improved Proliferation, Migration, and Therapeutic Potential

Figure 1

Comparative analysis of gene expression profiles of WJ-MSCs according to their proliferative capacity. (a) Doubling time of WJ-MSCs was measured, and the cells were classified into the proliferation-high (P-high) and proliferation-low (P-low) groups. (b) Heat map shows 33 differentially expressed genes related to cell differentiation, secretion, cell proliferation, cell adhesion, chemotaxis, and cell growth in both groups. (c) AURKA and DOCK2 genes expressed more significantly in the P-high group than in the P-low group. DEG selection criteria: significant fold changes (), fold , normalized read . (d) mRNA expression levels of AURKA and DOCK2 decreased at 48 h after siRNA transfection. (e) Suppression of AURKA and DOCK2 expression was examined by western blotting at 72 h after siRNA transfection. Normalized protein level indicates the value converted based on the siNC group. Data are shown as . Two-tailed Student’s -test (a, c). One-way ANOVA with Tukey’s multiple comparisons test (d, e). , , and .

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