Aloin Regulates Matrix Metabolism and Apoptosis in Human Nucleus Pulposus Cells via the TAK1/NF-κB/NLRP3 Signaling Pathway

Figure 5

Aloin suppresses the activation of the TAK1/NF-κB pathway. (a) Protein expression levels of the TAK1/NF-κB signaling pathway (TAK1, p-TAK1, IκBα, p-IκBα, P65, p-P65) were detected by western blotting in different groups. (b) The protein bands were quantified using the ImageJ software. (c) The expression ratios of p-TAK1/TAK1, p-IκBα/IκBα, and p-P65/P65 in the different groups. (d) Localization of P65 was assessed via immunofluorescence. Images (magnification: ×200, scale bar: 100 μm). In this figure, aloin was used at the concentration of 200 μM, while TNF-α was at the concentration of 10 ng/ml. , .

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