Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Figure 6

HUC-MSCs responded to the IL-1β microenvironment with the releasing of neovascularization factors to promote angiogenesis. (a) Quantitation of the relative gene expression of angiogenesis markers (bFGF, EGF, and HGF) in the hUC-MSCs with the various concentration of IL-1β induction. Data was reported as of 3 independent cultures, tested for significant difference (, , ns = nonsignificant) using Student’s -test. (b) Luminex analyzed the protein concentration of angiogenesis markers (bFGF, VEGF, and HGF) in the stimulated hUC-MSCs supernatant. Data was reported as of 3 independent cultures supernatant, tested for significant difference (, , ns = nonsignificant) using Student’s -test. (c) Phase contrast images of HUVEC cultured in the hUC-MSCs supernatant with or without the IL-1β induction.  μm. (d) Quantification of tube length of HUVEC culturing within the supernatant from the hUC-MSCs with or without the IL-1β induction. Results were presented as of approximately 60 pictures from 3 independent cultures, tested for significant difference () using Mann–Whitney rank sum test.

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