Extracellular Vesicles Secreted by TGF-β1-Treated Mesenchymal Stem Cells Promote Fracture Healing by SCD1-Regulated Transference of LRP5

Figure 7

SREBP-1 is required for SCD1 transcriptional response. (a) The SREBP-1 levels in MSCs^TGF-β1, MSC^TGF-β1-EVs, MSC^TGF-β1-EV-si-NC, or MSC^TGF-β1-EV-si-SREBP-1 were assessed by western blotting, . (b) MSCs were treated with TGF-β1, and nuclear SREBP-1 translocation was assessed by immunofluorescence, with quantification shown in the bar graph, . (c) The SCD1 protein levels in MSCs^TGF-β1 and MSC^TGF-β1-EVs treated with si-NC or si-SREBP-1 were assessed by western blotting, . (d) Schematics of the SCD1 promoter and luciferase construct are depicted with the binding site and deletion mutation sequences. (e) Relative luciferase activity of the WT SCD1 promoter or the MUT promoter was detected, . (F) ChIP assay shows binding of SREBP-1 to the SCD1 promoter is enhanced by TGF-β1 stimulation, . Data are expressed as ; and . ns: no significance; MSCs: mesenchymal stem cells; EVs: extracellular vesicles.

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