lncRNA CYTOR Facilitates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating SOX11 via Sponging miR-6512-3p

Figure 3

CYTOR facilitates osteogenic differentiation of PDLSCs via competitively binding to miR-6512-3p. (a) The miR-6512-3p expression was determined by qRT-PCR after transfection of miR-6512-3p mimic and miR-6512-3p inhibitor, compared with their respective control group. (b, c) The mRNA (b) and protein (c) levels of osteogenic related marker genes (RUXN2, Osterix, and OCN) were detected by western blot in indicated groups. (d, f) ALP staining (d) and ALP activity (f) in PDLSCs of indicated groups and cultured with OM for 7 days. (e) The formation of mineralized nodules was detected by Alizarin Red staining. (g) Semiquantification of (e). , , and , compared with the group as indicated.

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