lncRNA CYTOR Facilitates Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Modulating SOX11 via Sponging miR-6512-3p

Figure 4

SOX11 is upregulated during osteogenic differentiation of PDLSCs and is modulated by CYTOR and miR-6512-3p. (a) A Venn diagram of the intersections between the target genes of miR-6512-3p predicted on several online systems (TargetScan, StarBase, and miRDB) and the highly expressed genes from GO:0001649. (b, c). SOX11 mRNA (b) and protein (c) levels were determined by qRT-PCR and western blot during osteogenic differentiation of PDLSCs. (d) Effects of overexpression or knockdown of CYTOR on the protein level of SOX11. (e, f) The mRNA and protein levels of SOX11 were evaluated by qRT-PCR and western blot in PDLSCs after transfection of miR-6512-3p mimic/inhibitor or their respective control. (g) Diagrammatic sketch of the binding sequences for the wt (blue) and mut (red) of SOX11 mRNA 3 UTR associated with miR-6512-3p. (h) Luciferase activity of SOX11 mRNA 3 UTR wt/mut cotransfected with miR-6512-3p mimic or mimic NC. , , and . All tests were performed at least three times, and the values are presented as the .

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