Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT Signaling

Figure 3

Exogenous SMOC2 expression enhances BMP-9-induced late osteogenic marker expression and matrix mineralization. (a) Real-time PCR assay results showed the effect of SMOC2 on the mRNA expression of OPN promoted by BMP9 in MEFs. (b) Western blot assay results showed the effect of SMOC2 on the protein expression of OPN promoted by BMP9 in MEFs. (c) Quantification of the western blot results showed the effect of SMOC2 on OPN protein expression promoted by BMP9 in MEFs. (d) Real-time PCR assay results showed the effect of SMOC2 knockdown on the mRNA expression of OPN promoted by BMP9 in MEFs. (e) Western blotting assay results showed the effect of SMOC2 knockdown on OPN protein expression induced by BMP9 in MEFs. (f) Quantification of the western blot results showed the effect of SMOC2 on OPN protein expression promoted by BMP9 in MEFs. (g) Alizarin Red S staining results showed the effect of SMOC2 on BMP9-induced mineralization in MEFs. (h) Quantification results of Alizarin Red S staining showed the effect of SMOC2 on BMP9-induced mineralization in MEFs. (i) Alizarin Red S staining results showed the effect of SMOC2 knockdown on BMP9-induced mineralization in MEFs. (j) Quantification results of Alizarin Red S staining showed the effect of SMOC2 knockdown on BMP9-induced mineralization in MEFs. Compared with the control group, ; ; compared with the AdBMP9 group, #, ##.

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