Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT Signaling

Figure 4

The activation of FAK signaling in the enhancement effect of SMOC2 on BMP9-induced osteogenic differentiation in MEFs. (a) Western blot assay results showed that the levels of p-FAK and FAK were affected by BMP9 in MEFs at 30 h. (b) Quantification of the Western blot results showed that the levels of p-FAK and FAK were affected by BMP9. (c) ALP assay results showed the effect of FAK knockdown on the activity of ALP induced by BMP9 in MEFs. (d) Quantification results of the ALP assay showed the effect of FAK knockdown on the activity of ALP induced by BMP9. (e) Western blot assay results showed the effect of SMOC2 on the expression of p-FAK and p-AKT promoted by BMP9 in MEFs. (f–g) The quantification results of western blotting showed the effect of SMOC2 on the expression of p-FAK and p-AKT promoted by BMP9 in MEFs. (h) ALP assay results showed the reverse effect of SMOC2 on the effect of FAK knockdown on the BMP9-induced activity of ALP. (i) Quantification results of the ALP assay showed the reversal effect of SMOC2 on the effect of FAK knockdown on the BMP9-induced activity of ALP in MEFs. (j) Alizarin Red S staining showed the reversal effect of SMOC2 on the effect of FAK knockdown on BMP9-induced mineralization in MEFs. (k) Quantification results of Alizarin Red S staining showed the effect of SMOC2 on the effect of FAK knockdown on BMP9-induced mineralization in MEFs. Compared with the control group, ; ; compared with the AdBMP9 group, #, ##; compared with the AdBMP9 and siFAK group, ^, ^^.

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