Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids

Figure 5

Decreased number of AC3-positive ciliated RGCs in retinal organoids. (a) Representative images illustrating retinal organoids immunostained with neuronal cilia marker AC3 (gray), and prototypical cilia marker Arl13b (green). RGCs are visualized by Brn3b-tdTomato (red), and nuclei are stained with DAPI (blue). Primary cilia labeled with AC3 and/or Arl13b can be localized to Brn3b-positive RGCs (white arrows). (b, c) Quantification of the ratio of AC3-positive cilia with respect to the total number of Arl13b-positive cilia in Brn3b-positive RGCs (b) and non-RGCs (c). (d-f) Quantification of precent ciliated RGCs in human retina, day 176 and day 90 of retinal organoids. Values represent mean ± SD.

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