Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis

Figure 3

Effects of NEAT1 shuttled by ADSC-derived EVs on the biological properties of PCa cells. (a) Silencing efficiency of sh-NEAT1 in ADSCs detected by RT-qPCR. (b) NEAT1 expression in EVs from ADSCs transduced with sh-NEAT1-1 determined by RT-qPCR. (c) NEAT1 expression in SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs measured by RT-qPCR. (d) The expression of stemness genes (CD33, OCT-4, NANOG, and CD90) in SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs measured by RT-qPCR. (e) Clonogenic potential of SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs detected by colony formation assay. (f) Migration of SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs detected by Transwell assay. (g) Expression of EMT-related genes (E-cadherin, vimentin, and fibronectin) of SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs measured by RT-qPCR. (h) Resistance of SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs to gemcitabine detected by MTS assay. (i) Tumorigenicity of SW1990 cells in mice injected with SW1990 cells cocultured with EVs from sh-NEAT1-treated ADSCs (). vs. SW1990 cells transduced with sh-NC, SW1990 cells treated with PBS, or mice injected with SW1990 cells treated with PBS+DMSO or PBS+Gem; ^# vs. SW1990 cells cocultured with EVs from ADSCs transduced with sh-NC or mice injected with SW1990 cells treated with EVs+sh-NC+Gem. All data were presented as deviation. Comparisons of data between two groups were analyzed by unpaired -test, and comparisons of data among multiple groups were tested by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. Data at different time points were analyzed by repeated measurement ANOVA, followed by Tukey’s post hoc test. Cell experiments were independently repeated three times.

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