Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis

Figure 5

Effect of NEAT1 and miR-491-5p on PCa cell activities and gemcitabine resistance. SW1990 cells were transduced with overexpression vector-mediated NEAT1 alone or combined with miR-491-5p. (a) Expression of NEAT1 and miR-491-5p determined by RT-qPCR. (b) Clonogenic potential of SW1990 cells detected by colony formation assay. (c) Migration of SW1990 cells detected by Transwell assay. (d) Expression of EMT-related genes (E-cadherin, vimentin, and fibronectin) of SW1990 cells measured by RT-qPCR. (e) Resistance of SW1990 cells to gemcitabine detected by MTS assay. (f) Tumorigenicity of SW1990 cells in mice (). vs. SW1990 cells transduced with Vector+NC mimic or Vector+mimic NC+DMSO, or mice injected with SW1990 cells treated with Vector+NC mimic; ^# vs. SW1990 cells transduced with NEAT1+NC mimic or NEAT1+mimic NC+Gem, or mice injected with SW1990 cells treated with NEAT1+NC mimic. All data were presented as deviation. Comparisons of data among multiple groups were tested by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. Data at different time points were analyzed by repeated measurement ANOVA, followed by Tukey’s post hoc test. Cell experiments were independently repeated three times.

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