Molecular Mechanism of Long Noncoding RNA SNHG14 in Osteogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells through the NEDD4L/FOXA2/PCP4 Axis

Figure 6

FOXA2 downregulation attenuates the inhibition of silencing SNHG14 on OD of BMSCs. BMSCs were infected with FOXA2 shRNA lentivirus (sh-FOXA2), with sh-NC as the control. (a, b) FOXA2 expression level was determined by RT-qPCR and Western blot analysis. (c) ALP staining and ALP activity determination. (d) Alizarin red staining and determination of mineralized nodules. (e) Expression levels of OCN and OPN were determined by Western blot analysis. Cell experiments were repeated 3 times. Data were represented as deviation. Multigroup comparisons in panels (c) and (d) were analyzed using one-way ANOVA and in panel (e) were analyzed using two-way ANOVA, followed by Tukey’s multiple comparison test. Pairwise comparisons in panels (a) and (b) were analyzed using the -test. , .

(a)

(b)

(c)

(d)

(e)