EVL Promotes Osteo-/Odontogenic Differentiation of Dental Pulp Stem Cells via Activating JNK Signaling Pathway

Figure 2

Knockdown of EVL inhibited the osteo-/odontogenic differentiation of hDPSCs. (a, b) The expression of EVL during osteo-/odontogenic differentiation was confirmed with qRT-PCR and western blot. (c, d) The knockdown efficiencies of siRNAs against EVL were verified by western blot assay and qRT-PCR. (e) The expressions of DSPP, DMP1, RUNX2, OCN, and OSX were analyzed in hDPSCs transfected with si-EVL and si-NC by western blot on the 7th day. (f) The expressions of DSPP, DMP1, ALP, RUNX2, OSX, OCN, and COL1A1 were analyzed in hDPSCs transfected with si-EVL and si-NC by qRT-PCR on the 3rd and 7th day. (g) ALP staining and quantitative intracellular ALP activity were measured in hDPSCs transfected with si-EVL and si-NC and cultured in growth medium (GM) or osteogenesis-inducing medium (OM) on the 3rd and 7th day. (h) Extracellular calcium deposition was visualized by alizarin red staining on the 21st day. Released dye in alizarin red staining was quantified by measuring absorbance at 562 nm. GAPDH was used as an internal control in qRT-PCR and western blot analyses. Data are shown as the (); , , .

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