Mechanisms for Covalent Immobilization of Horseradish Peroxidase on Ion-Beam-Treated Polyethylene

Table 2

Amount of HRP attached protein (see (5)) on untreated and PIII-treated UHMWPE and on copolymer of polyethylene and methancrylic acid (co-PE-MMA) before and after washing in detergents. The HRP protein attachment was done on PIII-treated sample in air, in nitrogen at low pressure (30 Torr), and high pressure (760 Torr). Carbonyl group relative concentration is measured by intensity ratio of 1691 cm⁻¹ line and 1462 cm⁻¹ line in FTIR ATR spectra. The average error bar for the protein amount is 0.0010.

Sample description	Amount of attached after incubation	Amount after SDS	Amount after Triton	Amount after Tween 20	C=O concentration before attachment
Untreated	0.0087	0.0041	0.0018	0.0023	~0
PIII, in air	0.0198	0.0143	0.0142	0.0167	0.14
PIII, in N2, 30 Torr	0.0182	0.0161	0.0106	0.0100	~0
PIII, in N2, 760 Torr	0.0255	0.0147	0.0186	0.0180	~0
co-PE-MMA, pressed	0.0086	0	0.0033	0.0024	0.46
co-PE-MMA, pressed and ethanol washed	0.0057	0	0.0014	0.0029	0.53