Research Article

Feminization of Longnose Dace (Rhinichthys cataractae) in the Oldman River, Alberta, (Canada) Provides Evidence of Widespread Endocrine Disruption in an Agricultural Basin

Figure 5

Box plots illustrating normalized liver vitellogenin mRNA expression in male (a) and female (b) longnose dace (Rhinichthys cataractae) from the Oldman River, Alberta sampled during August and September 2009. Vitellogenin mRNA expression is normalized to GAPDH. There were insufficient males collected from Highway 3A to analyze male vitellogenin mRNA. The box plot labeled “reference” is for dace collected at Popson Park and reared in the laboratory for 7 months in clean water and commercial food.
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