The Reporter System for GPCR Assay with the Fission Yeast Schizosaccharomyces pombe
The schematic illustration of reporter plasmid construction. (a) Ligation of the fragment amplified from pAL7 and the phosphated fragment including a series of promoter, GFP (filled arrow) and LPI terminator (filled box). (b) Conversion of the promoter into any upstream regions (shaded and open triangles), and conversion of the LPI terminator into any downstream regions (shaded and open boxes). (c) Completion of converting upstream and downstream region. All reporter plasmids listed in Table 1 were constructed in this way.