The BRCA1 Breast Cancer Suppressor: Regulation of Transport, Dynamics, and Function at Multiple Subcellular Locations
Figure 2
BRCA1 targeting to centrosomes and nuclear foci. (a) Deletion mapping studies identified the same minimal targeting sequences (the amino and carboxy termini combined) that were required and sufficient to target BRCA1 to nuclear foci [45] and the centrosome [46]. (b) This is illustrated by a summary diagram. (c) BRCA1 is localized to nuclear foci when analysed by immunofluorescence microscopy. The BRCA1 staining correlates with large visible nuclear spots thought to be DNA replication sites in untreated S-phase cells (see -IR cell images), and redistributes to smaller DNA repair foci after 3 h treatment with 10 Gy ionising radiation (+IR). The images shown are of a single cell nucleus and reveal the typical costaining observed between BRCA1 (green) and BARD1 (red), with overlap displayed as yellow (left-hand image). The overlap in staining is also visible in a three-dimensional rotation of the images (right-hand image; adapted from [47]).
