An Elegant Biosensor Molecular Beacon Probe: Challenges and Recent Solutions
Figure 9
Strategies to reduce the background fluorescence of MB probes. (a) Superquenched MB probe contains more than one quencher [75]. (b) In-stem probe takes advantage of stacking interaction of stem base pairs with a fluorophore and a quencher dyes to achieve efficient contact quenching [110, 111]. (c) Gold nanoparticle-conjugated MB probe takes advantage of superior quenching abilities of gold nanoparticles [74]. (d) Triplex-forming MB probe makes use of more than one quencher [112] to reduce the background fluorescence.
