Genes Encoding Toxin of Clostridium difficile in Children with and without Diarrhea

Table 1

Genes and primer/probe sets used to detect C. difficile and genes encoding toxins.

Genes	Oligonucleotides; 5′→3′	Melting temperature (°C)	References
16S rRNA∗	F-TTG AGC GAT TTA CTT CGG TAA AGA; R-TGT ACT GGC TCA CCT TTG ATA TTC A; P-CCA CGC GTT ACT CAC CCG TCC G	60	[16]
𝑡𝑐𝑑𝐴^∗∗	F-CAG GGC TAA TAG TTT GTT TAC AGA ACA; R-CAA CAT CTA AAT ATA CTC CGC CAA AA; P-TTA TAG TCA GCA GCT AGG ATT TCC ACG ATT TAA CAA CTC C	51	[17]
𝑡𝑐𝑑𝐵^∗∗	F-AGC AGT TGA ATA TAG TGG TTT AGT TAG AGT TG; R-CAT GCT TTT TTA GTT TCT GGA TTG AA; P-CAT CCA GTC TCA ATT GTA TAT GTT TCT CCA	58	[17]
𝑐𝑑𝑡𝐴^∗∗	F-GAT CTG GTC CTC AAG AAT TTG GTT; R-GCT TGT CCT TCC CAT TTT CGA TT; P-AAC TCT TAC TTC CCC TGA AT	51	[17]
𝑐𝑑𝑡𝐵^∗∗	F-AAA AGCT TCA GGT TCT TTT GAC AAG; R-TGA TCA GTA GAG GCA TGT TCA TTT G; P-CAA GAG ATC CGT TAG TTG CAG CAT ATC CAA TTG T	58	[17]

∗Used to detect C. difficile.
∗∗Used to detect toxigenic C. difficile.