Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study

Table 12

Advantages of XF H and E method.

	Routine H and E	1.5% DWS	95% DLW
Cost	High	Low	Low
Time	50–55 min	25–30 min	54 min
Toxicity	Present	Absent	Absent
Biohazardous	Yes	No	No
Inflammability of chemicals used	Present	Absent	Absent
Staining protocol	Lengthy	Simplified	Simplified
Handling	Toxic if not properly handled	Easy	Easy
Quality of staining	Good	Good	Good
Disposal of chemicals	Difficult	Easy	Easy
Preparation	Synthetic	Synthetic	Naturally available from plants