Efficacy of 1.5% Dish Washing Solution and 95% Lemon Water in Substituting Perilous Xylene as a Deparaffinizing Agent for Routine H and E Staining Procedure: A Short Study

Table 2

Routine H and E staining procedure.

Deparaffinization and Rehydration	Xylene	25 min
	90% alcohol	1 min
	70% alcohol	1 min
	Water wash	1 min
Nuclear staining	Harris hematoxylin	8 min
	Tap water wash	2 min
Differentiation	Differentiation in 1% acid alcohol	1 dip
Bluing	1% lithium carbonate	1 min
	Water wash	10 min
Cytoplasmic staining	1% eosin	1 min
Dehydration	70% alcohol	30 sec
	90% alcohol	30 sec
	Xylene	1 min
	Approximate time required	50 to 55 min