Figure 5: Ice recrystallization inhibition by antifreeze proteins. Flash frozen samples are held at −6°C for 60 minutes. Following this wait, ice crystal size at both time points are compared. Top figures show large recrystallized ice using a control solution, consisting of 50 mM Tris (pH 8.0) and 50 mM NaCl. Bottom figures show ice recrystallization inhibition using an antifreeze protein solution containing 0.1 mg/mL AfpA in control buffer.