Chloroplast DNA Variations in Wild Brassicas and Their Implication in Breeding and Population Genetics Studies

Table 2

PCR conditions and size of amplified fragments in wild brassicas using six universal primer pairs.

Abbreviation of cpDNA primers^a	PCR conditions		Size of amplified fragment (bp)
	Annealing temperature	Extension time
CD	52°C	4 min 30 sec	2400
DT	52°C	2 min	1200
HK	51°C	2 min 30 sec	1750
K1K2	53°C	3 min	2600
TF	50°C	2 min 30 sec	1700
VL	54°C	4 min 30 sec	3800

Abbreviation and primer sequences as in Dumolin-Lapegue et al. (1997) [12].