Table 3: Optimized component concentrations for PCR.

ComponentsConcentrationVolume/reaction

PCR buffer with MgCl210x2.5 L
dNTPs2 mM2.5 L
Forward primer10 pm/L1 L
Reverse primer10 pm/L1 L
Taq polymerase3 U/L0.4 L
PCR water15.6 L
Genomic DNA50 ng/L2 L
Total25 L