KCl-Dependent Release of Mitochondrial Membrane-Bound Arginase Appears to Be a Novel Variant of Arginase-II

Figure 5

Data showing the activity of arginase under influence of increasing KCl concentration on isolated mitochondrial fraction in experiment set 2 in which mitochondria were isolated first and then washed with different concentration of KCl in homogenizing buffer. (a) Graph shows decreasing activity of arginase with the increasing concentration of KCl in the homogenizing buffer. Data is mean of three experiments and histogram represents mean ± SD; . (b) The mitochondrial matrix fraction probed against anti-arginase-II cross reacts with single band of 40 kDa. The intensity of band was decreasing with increasing concentration of KCl. Cytochrome C (Cyt C) was taken as loading control. (c) The increased activity of arginase in membrane-bound fraction was observed with increasing concentration of KCl in isolation buffer; . (d) The membrane-bound fraction probed against anti-arginase-II has shown band of 40 kDa which is expected for anti-arginase-II along with the band of 50 kDa that shows progressive increase with increasing concentration of KCl;The variant that is immunoreactive with anti-arginase-II. Cytochrome C (Cyt C) was taken as loading control.

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