A High-Fat, High-Fructose Diet Induces Antioxidant Imbalance and Increases the Risk and Progression of Nonalcoholic Fatty Liver Disease in Mice

Table 2

PCR programs for mouse CAT, CuZn-SOD, Mn-SOD, GPx, and GAPDH.

Gene	Denaturation (°C/sec)	Annealing (°C/sec)	Extension (°C/sec)	Number of cycles	Product size (bp)	References
CAT	94/45	55/60	72/60	31	229	[17]
CuZn-SOD	94/45	56/60	72/60	26	246	[18]
Mn-SOD	94/45	55/60	72/60	35	241	[18]
GPx	94/45	56/60	72/60	28	197	[18]
GAPDH	95/30	64/30	72/60	28	145	[19]