[Retracted] A Simple HPLC-UV Method for the Determination of Glutathione in PC-12 Cells

Figure 3

Representative chromatogram from 6 replicates is shown. (a) 2-Nitro-5-mercapto-benzoic (NMB) acid, Peak 1 with RT 7.43 min; glutathione dimer (GSH Dimer), Peak 2 with RT 10.93 min, and Ellman’s reagent (DTNB), Peak 3 RT 14.12 min in untreated PC-12 cells (control). (b) NMB, Peak 1 with RT 7.44 min, GSH Dimer, Peak 2 with RT 10.88 min, and DTNB, Peak 3 retention time 14.10 min in PC-12 cells treated with methylglyoxal. (c) NMB, Peak 1 with RT 7.59 min, glutathione dimer, Peak 2 with RT 10.92 min, and DTNB, Peak 3 RT 14.44 min in PC-12 cells treated with CoCl₂.

(a)

(b)

(c)