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Scientifica
Volume 2016 (2016), Article ID 9020239, 7 pages
http://dx.doi.org/10.1155/2016/9020239
Research Article

Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium Inhabiting Euphorbia hirta L. and Their Bactericidal Potential

1Bionanotechnological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore, Karnataka 570006, India
2Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Technical Institutional Campus, Mysore 570006, India
3Division of Microbiology, Faculty of Life Sciences, JSS University, Mysore, India
4Department of Zoology, Bangalore University, Jnanabharathi Campus, Bangalore, Karnataka 560056, India

Received 31 December 2015; Accepted 4 May 2016

Academic Editor: Joaquim Ruiz

Copyright © 2016 Baker Syed et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θ which can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among which Pseudomonas aeruginosa was most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabiting Euphorbia hirta L. in mediating synthesizing silver nanoparticles.