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Volume 2017, Article ID 7616359, 10 pages
Research Article

Comparative Metagenomic Analysis of Electrogenic Microbial Communities in Differentially Inoculated Swine Wastewater-Fed Microbial Fuel Cells

1Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, Russia
2Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, Russia
3Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia
4Biological Systems Unit, Okinawa Institute of Science and Technology, Okinawa, Japan
5Department of Biology, Sonoma State University, Rohnert Park, CA, USA
6School of Informatics, University of Edinburgh, Edinburgh, UK
7Tianjin Institute of Industrial Biotechnology, Tianjin, China

Correspondence should be addressed to Irina V. Khilyas; moc.liamg@saylihk.aniri

Received 24 April 2017; Accepted 15 August 2017; Published 12 October 2017

Academic Editor: Ravinder Malik

Copyright © 2017 Irina V. Khilyas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: Cell voltage and power density vs. current density (cell polarization) of MFCs (A) inoculated with swine sludge (SS); (B) inoculated with industrial granular brewery sludge (IGSB). Open circles, voltage; closed circles, power density.

Figure S2: Current generation during swine wastewater treatment by MFCs inoculated with swine waste sludge and brewery sludge. Mean data from duplicate experiments; error bars indicating ±SD to not exceed the diameter of the data point symbols. Open boxes, SS-inoculated MFC; open diamonds, IGBS-inoculated MFC.

Figure S3: Total COD concentrations in SW feed and within MFCs inoculated with swine waste sludge and industrial granular brewery sludge. Squares, SS-inoculated MFC; circles, IGBS-inoculated MFC; Mean data from duplicate experiments.

Figure S4: Change in total phosphorus (PO43-P) in inflow and outflows of MFCs inoculated with swine wastewater sludge (SS) and industrial granular brewery sludge (IGBS). Mean data from duplicate experiments with error bars (±SD).

Figure S5: Changes in ammonia nitrogen (NH4-N) in inflow and outflows of MFCs inoculated with swine wastewater sludge (SS) and industrial granular brewery sludge (IGBS). Mean data from duplicate experiments with error bars (±SD).

Figure S6: SEM images of the anodic biofilms the MFCs inoculated with (A) swine waste and (B) brewery sludge. Samples of anode surfaces (activated carbon granules and fiber) the MFCs were taken after 67 days of swine wastewater treatment upon disassembling the MFCs. Slices of anode electrodes (1 cm2) were briefly rinsed with deionized water and fixed in 2.5% glutaraldehyde for 2 h, further in 1% osmium tetroxide. Dehydration of microbial biofilms was carried out using a series of ethanol–water solutions (25, 50, 75, 95, 100%). After gold coating, the obtained specimens were observed using a Focused Ion Beam Scanning electron microscope (Helios NanoLab 650, USA). High resolution images were acquired using an accelerating voltage of 20 kV at a working distance of 3.1–6.5 mm.

Table S1: Summary of MFC operation modes.

Table S2: VFA concentrations in SS-inoculated MFCs.

Table S3: VFA concentrations in IGBS-inoculated MFCs.

Table S4: Diversity of dominated species in the SW, inocula, anodic and planktonic microbial communities of SS- and IGBS-inoculated MFCs.

  1. Supplementary Material