Scientifica / 2018 / Article / Fig 8

Research Article

Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Figure 8

The effect of bacterial inoculum size on glucose isomerase production. In this study, we determined the optimal bacterial inoculum size is 20%. Addition of bacteria (5–20%) in induction medium resulted an increase of enzyme activity, but bacterial inoculum size also exhibited the inhibitory effect at the higher levels (25–30%). a, b, c, and d indicate the significant difference between groups.