Scientifica / 2018 / Article / Tab 1

Research Article

Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Table 1

Plackett–Burman experimental design matrix for screening variables having significant impact for glucose isomerase production by Bacillus megaterium.

RunsVariablesEnzymatic activity (U/mg biomass)
X1X2X3X4X5X6X7X8X9

11.50.10.150.750.56.525391400.207
21.50.30.051.250.56.515391400.221
30.50.30.150.751.56.515351400.206
41.50.10.151.250.57.515351000.263
51.50.30.051.251.56.525351000.271
61.50.30.150.751.57.515391000.181
70.50.30.151.250.57.525351400.237
80.50.10.151.251.56.525391000.195
90.50.10.051.251.57.515391400.208
101.50.10.050.751.57.525351400.243
110.50.30.050.750.57.525391000.132
120.50.10.050.750.56.515351000.184

Variables are encoded as follows: D-xylose concentration (X1), K2HPO4 concentration (X2), MgSO4·7H2O concentration (X3), yeast extract concentration (X4), peptone concentration (X5), initial pH (X6), bacterial inoculum size (X7), cultivation temperature (X8), and rotation rate (X9).