Scientifica / 2018 / Article / Tab 1

Research Article

Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology

Table 1

Plackett–Burman experimental design matrix for screening variables having significant impact for glucose isomerase production by Bacillus megaterium.

RunsVariablesEnzymatic activity (U/mg biomass)

Variables are encoded as follows: D-xylose concentration (X1), K2HPO4 concentration (X2), MgSO4·7H2O concentration (X3), yeast extract concentration (X4), peptone concentration (X5), initial pH (X6), bacterial inoculum size (X7), cultivation temperature (X8), and rotation rate (X9).