In Vitro Seed Germination and Seedling Development of a Rare Indonesian Native Orchid Phalaenopsis amboinensis J.J.Sm

Figure 2

In vitro seed culture and propagation of P. amboinensis J.J.Sm. (A) Mature seed under scanning electron microscopy; (B) stage 0, no germination, long section of viable seed in elliptical embryo shape; (C) stage 1, germination, swollen embryo; (D) stage 2, embryo is completely discharged from the testa; (E) stage 3, embryo with pointed shoot apex; (F) stage 4, appearance of absorbing hair and emergence of leaves; (G) stage 5, seedling stage, root with leaves; (H) in vitro seed culture and protocorm development on VW medium eight weeks after inoculation; (I) seedlings in vitro on VW medium supplemented with coconut water 15% (v/v) and banana homogenate 10 g·L⁻¹; (J) plantlet development on 12 weeks after transplanted to plastic pots. e: embryo; t: testa; sa: shoot apex; ah: absorbing hair; fl: first leaf; sl: second leaf; r: root. Scale bars: (A, B) 100 μm, (C) 140 μm, (D) 840 μm, (E) 1900 μm, (F) 3.6 mm, (G) 0.9 mm, (H, I) 1.2 cm, and (J) 1.4 cm.